| 货号 | 93421S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse |
| 应用 | IF,F |
| 目标/特异性 | iNOS (D6B6S) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total iNOS protein. This antibody does not cross-react with other NOS proteins. |
| 使用方法 | Immunofluorescence (Immunocytochemistry) (1:50) Flow Cytometry (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Nitric Oxide Synthase (NOS) catalyzes the formation of nitric oxide (NO) and citruline from L-arginine, oxygen and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3). |
| 存放说明 | 4C |
| 参考文献 | 1 . Tsutsui, M. (2004) J Atheroscler Thromb 11, 41-48. 2 . Son, H. et al. (1996) Cell 87, 1015-23. 3 . Hawkins, R.D. (1996) Neuron 16, 465-467. |
Confocal immunofluorescent analysis of Raw cells, untreated (left), or treated with Lipopolysaccharides (LPS) #14011 (1 μg/ml, 16 hrs; right) using iNOS (D6B6S) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of Raw 309 Cr.1 cells, untreated (blue) or Lipopolysaccharides (LPS) #14011 treated (1 μg/ml, overnight; green), using iNOS (D6B6S) Rabbit mAb (Alexa Fluor®488 Conjugate). |
