| 货号 | 87995S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | F |
| 目标/特异性 | Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of IRAK4 protein only when phosphorylated at Thr345 and Ser346. This antibody does not react with single phosphorylated IRAK4. |
| 使用方法 | Flow Cytometry (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88, and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination, and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm where it activates protein kinase cascades, including TAK1, IKKs, and the stress-activated kinases (3). |
| 存放说明 | 4C |
| 参考文献 | 1 . Dinarello, C.A. (1996) Blood 87, 2095-147. 2 . Takaesu, G. et al. (2001) Mol Cell Biol 21, 2475-84. 3 . Janssens, S. and Beyaert, R. (2003) Mol Cell 11, 293-302. |
Flow cytometric analysis of KARPAS-299 cells, untreated (blue) or treated with Human Interleukin-1β (hIL-1β) #8900 (50 ng/ml, 15 min) (green), using Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb (Alexa Fluor® 647 Conjugate). KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge. |
