| 货号 | 74244S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | F |
| 目标/特异性 | Estrogen Receptor α (D6R2W) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total estrogen receptor α protein. |
| 使用方法 | Flow Cytometry (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Estrogen receptor α (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity (3-5). Ser104, 106, 118, and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). According to the research literature, phosphorylation at Ser167 may confer tamoxifen resistance in breast cancer patients (4). |
| 存放说明 | 4C |
| 参考文献 | 1 . Mangelsdorf, D.J. et al. (1995) Cell 83, 835-9. 2 . Glass, C.K. and Rosenfeld, M.G. (2000) Genes Dev 14, 121-41. 3 . Chen, D. et al. (1999) Mol Cell Biol 19, 1002-15. 4 . Campbell, R.A. et al. (2001) J Biol Chem 276, 9817-24. 5 . Chen, D. et al. (2000) Mol Cell 6, 127-37. 6 . Joel, P.B. et al. (1998) Mol Cell Biol 18, 1978-84. |
Flow cytometric analysis of MDA-MB-231 (blue) and MCF7 (green) cells using Estrogen Receptor α (D6R2W) Rabbit mAb (PE Conjugate). |
