| 货号 | 25678S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse |
| 应用 | F |
| 目标/特异性 | Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of PLCγ1 protein only when phosphorylated at Tyr783. |
| 使用方法 | Flow Cytometry (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (2). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197, and 1217 is correlated with PLCγ2 activity (5,6). |
| 存放说明 | 4C |
| 参考文献 | 1 . Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509. 2 . Margolis, B. et al. (1989) Cell 57, 1101-7. 3 . Kim, H.K. et al. (1991) Cell 65, 435-41. 4 . Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7. 5 . Watanabe, D. et al. (2001) J Biol Chem 276, 38595-601. 6 . Ozdener, F. et al. (2002) Mol Pharmacol 62, 672-9. |
Flow cytometric analysis of serum-starved NIH/3T3 cells, untreated (blue) or treated with mouse platelet-derived growth factor BB (200 ng/ml, 15 min; green), using Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb (Alexa Fluor® 488 Conjugate) (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (dashed lines). |
