| 货号 | 50230S |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | All |
| 来源宿主 | Mouse IgG1 |
| 应用 | F |
| 目标/特异性 | BrdU (Bu20a) Mouse mAb (PE Conjugate) detects BrdU when incorporated into single stranded DNA. DNA must be denatured for the epitope to be exposed and recognized by the antibody. |
| 使用方法 | F |
| 供应商 | CST |
| 背景 | Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine (BrdU) are useful for labeling nascent DNA in living cells and tissues. BrdU becomes incorporated into replicating DNA in place of thymidine and subsequent immunodetection of BrdU using specific monoclonal antibodies allows labeling of cells in S phase of the cell cycle. After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb can be used to detect BrdU incorporated into single stranded DNA. Please see our detailed protocol for information regarding the labeling procedure and denaturation of double stranded DNA for various immunodetection applications (1-4). |
| 存放说明 | 4C |
| 参考文献 | 1 . Darzynkiewicz, Z. and Juan, G. (2001) Curr Protoc Cytom Chapter 7, Unit 7.7. 2 . Leif, R.C. et al. (2004) Cytometry A 58, 45-52. 3 . Staszkiewicz, J. et al. (2009) Biochem Biophys Res Commun 378, 539-44. 4 . Rothaeusler, K. and Baumgarth, N. (2007) Curr Protoc Cytom Chapter 7, Unit7.31. |
Flow cytometric analysis of Jurkat cells incorporated with BrdU (30 min), using BrdU (Bu20a) Mouse mAb (PE conjugate) versus DRAQ5® (DNA content). |
