| 货号 | 53496S |
| 同种亚型 | Mouse IgG2a |
| 反应种属 | Human\Mouse\Rat\Moneky |
| 来源宿主 | Mouse IgG2a |
| 应用 | F |
| 目标/特异性 | Lamin A/C (4C11) Mouse mAb (PE Conjugate) detects endogenous levels of lamin A and lamin C proteins. It also reacts with the larger fragments of lamin A (50 kDa) and lamin C (41 kDa) produced by caspase cleavage during apoptosis. This antibody does not cross-react with lamins B1 and B2. |
| 使用方法 | F |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear dysregulation and cell death (5,6). |
| 存放说明 | 4C |
| 参考文献 | 1 . Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23. 2 . Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84. 3 . Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93. 4 . Orth, K. et al. (1996) J Biol Chem 271, 16443-6. 5 . Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37. 6 . Rao, L. et al. (1996) J Cell Biol 135, 1441-55. |
Flow cytometric analysis of HeLa cells using Lamin A/C (4C11) Mouse mAb (PE Conjugate) (green) compared to #5742 Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) (red). |
