| 货号 | 94794S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | F, IF-IC |
| 目标/特异性 | Sox9 (D8G8H) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total Sox9 protein. |
| 使用方法 | IF-IC F |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Sox9 is a transcription factor with an HMG-box DNA binding domain that has homology to the HMG domain of the mammalian testis-determining factor, SRY (1). Sox9 regulates several important processes during embryonic development including chondrogenesis, during which it contributes to skeletal formation and digit specification (2,3). Sox9 also coordinates with steroidogenic factor-1 to direct Sertoli cell-specific expression of anti-Mullerian hormone during embryogenesis, thereby contributing to male sex determination (4). In addition, Sox9 is reportedly involved in the maintenance of adult stem cell populations, including multipotent neural stem cells (5), hair follicle stem cells (6), and mammary stem cells (7). Recent interest has focused on the role of Sox9 in tumor biology. For example, research studies have shown that Sox9 expression in lung adenocarcinoma induces a mesenchymal phenotype in tumor cells (8). Other research studies have shown that YAP1 induced upregulation of Sox9 confers cancer stem cell like properties on esophageal cancer cells (9). Moreover, Sox9 expression has been linked with several other tumor types including ovarian, prostate, and pancreatic malignancies (10-12). |
| 存放说明 | 4C |
| 参考文献 | 1 . Mertin, S. et al. (1999) Nucleic Acids Res 27, 1359-64. 2 . Akiyama, H. et al. (2002) Genes Dev 16, 2813-28. 3 . Raspopovic, J. et al. (2014) Science 345, 566-70. 4 . De Santa Barbara, P. et al. (1998) Mol Cell Biol 18, 6653-65. 5 . Scott, C.E. et al. (2010) Nat Neurosci 13, 1181-9. 6 . Kadaja, M. et al. (2014) Genes Dev 28, 328-41. 7 . Guo, W. et al. (2012) Cell 148, 1015-28. 8 . Capaccione, K.M. et al. (2014) Oncotarget 5, 3636-50. 9 . Song, S. et al. (2014) Cancer Res 74, 4170-82. 10 . Raspaglio, G. et al. (2014) Gene 542, 173-81. 11 . Qin, G.Q. et al. (2014) Onco Targets Ther 7, 137-46. 12 . Shroff, S. et al. (2014) Hum Pathol 45, 456-63. |
Flow cytometric analysis of HeLa (blue) and A-204 (green) cells using Sox9 (D8G8H) Rabbit mAb (Alexa Fluor® 488 Conjugate). | |
Confocal immunofluorescent analysis of A-204 (left) or HeLa (right) cells using Sox9 (D8G8H) (Alexa Fluor® 488 Conjugate) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). |
