β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate)
货号:
2116S 基本售价:
4060.0 元 规格:
-
产品信息
概述货号 | 2116S |
描述 | Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for direct immunofluorescent analysis of human and monkey cells. The unconjugated antibody #2128 reacts with human, mouse, rat, monkey, bovine, zebrafish and fly β-tubulin protein. CST expects that β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) will also recognize β-tubulin in these species. |
反应种属 | Human/Mouse/Rat/Monkey/Zebrafish/Bovine |
来源宿主 | Rabbit |
应用 | IF-IC |
目标/特异性 | β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of total β-tubulin protein. |
使用方法 | IF-IC(1:200) |
性能供应商 | CST |
标记 | Alexa Fluor 555 |
背景 | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
存放说明 | 4C |
参考图片Confocal immunofluorescent analysis of HeLa cells, serum-starved (left) or 20% serum-treated (right), using β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) (red) and Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) (红色)和Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854 (绿色)标记,共聚焦免疫荧光观察β-Tubulin和Phospho-S6 Ribosomal Protein蛋白在HeLa细胞中定位,细胞分为serum-starved (左图) or 20% serum-treated (右图)。蓝色伪彩=DRAQ5®#4084(DNA荧光染料)。 |