VEGF Receptor 2 (55B11) Rabbit mAb (Sepharose Bead®Conjugate)
货号：
5168S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 5168S |
| 描述 | This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. VEGF Receptor 2 (55B11) Rabbit mAb (Sepharose Bead Conjugate) is useful for immunoprecipitation assays. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated VEGF Receptor 2 (55B11) Rabbit mAb #2479. |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | IP |
| 目标/特异性 | VEGF Receptor 2 (55B11) Rabbit mAb (Sepharose Bead Conjugate) detects endogenous levels of VEGF receptor 2 protein. This antibody does not cross-react with other family members. |
| 使用方法 | IP(1:20) |
性能| 供应商 | CST |
| 标记 | Sepharose Bead |
| 背景 | Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, Grb2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 210, 230 |
参考图片Immunoprecipitation of PAEC/CKR cell lysates using VEGF Receptor 2 (55B11) Rabbit mAb (Sepharose Bead Conjugate) and Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423. The western blot was probed using VEGF Receptor 2 (55B11) Rabbit mAb #2479. PAEC/CKR cells overexpress chimeric receptors containing the human CSF-1 extracellular binding domain/mouse VEGF receptor 2 intracellular domains (7).用VEGF Receptor 2 (55B11) Rabbit mAb (Sepharose Bead Conjugate)和Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423对PAEC/CKR细胞的裂解液进行免疫共沉淀。蛋白质印记检测使用VEGF Receptor 2 (55B11) Rabbit mAb #2479检测。PAEC/CKR 细胞高表达含有人的CSF-1胞外结合区/小鼠VEGF receptor 2胞内区的嵌合受体 (7)。 |
