SV40 Large T Antigen (D1E9E) Rabbit mAb (Sepharose® Bead Conjugate)
货号：
45159S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 45159S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | All Species Expected |
| 应用 | IP |
| 目标/特异性 | SV40 Large T Antigen (D1E9E) Rabbit mAb (Sepharose® Bead Conjugate) recognizes transgenic levels of total SV40 Large T Antigen protein. |
| 使用方法 | Immunoprecipitation (1:20)
|
性能| 供应商 | CST |
| 灵敏度 | Recombinant |
| 背景 | Simian Virus 40 (SV40) is a small double-stranded, circular DNA tumor virus encoding three tumor antigens: large T, Small T, and 17KT. These antigens contribute to cellular transformation, an area of research where they are utilized extensively. Transformation of cells by SV40 large T antigen is mediated by its binding to the tumor suppressor proteins RB and p53 in the nucleus. Additional binding partners include p300, CBP, Cul7, IRS1, Bub1, Nbs1, and Fbw7, which also contribute to transformation (1). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
| 参考文献 | 1 . Cheng, J. et al. (2009) Semin Cancer Biol 19, 218-28.
|
参考图片Immunoprecipitation of extracts from 293 cells (lane 1 and 3) or 293T cells (lane 2 and 4) using Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose® Bead Conjugate) #3423 (lane 1 and 2) or SV40 Large T Antigen (D1E9E) Rabbit mAb (Sepharose® Bead Conjugate) (lane 3 and 4). Lanes 5 and 6 are 10% input. Western blot analysis was performed using SV40 Large T Antigen (D1E9E) Rabbit mAb #15729 as the primary antibody and was visualized using Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127. |
