| 货号 | 87593S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat |
| 应用 | F |
| 目标/特异性 | Phospho-SAMHD1 (Thr592) (D7O2M) Rabbit mAb (PE Conjugate) recognizes endogenous levels of SAMDH1 protein only when phosphorylated at Thr592. |
| 使用方法 | Flow Cytometry (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SAM domain and HD domain-containing protein 1 (SAMHD1) is a negative regulator of the cell-intrinsic innate immune response (1). Research studies have identified mutations in SAMHD1 as a cause of Aicardi-Goutieres syndrome, an autoimmune disease characterized by elevated production of interferon-α and symptoms resembling congenital viral infection (1). SAMHD1 was identified as the restriction factor that renders most myeloid cells refractory to human immunodeficiency virus (HIV) infection (2-4). Expression of the viral protein Vpx in refractory cells targets SAMHD1 for ubiquitin-mediated degradation and relieves HIV restriction. SAMHD1 prevents autoimmunity and HIV infection by hydrolyzing intracellular deoxynucleoside triphosphates (dNTPs), thereby limiting inappropriate immune activation by self nucleic acid and inhibiting reverse transcription of the HIV genome (4-6). |
| 存放说明 | 4C |
| 参考文献 | 1 . Rice, G.I. et al. (2009) Nat Genet 41, 829-32. 2 . Laguette, N. et al. (2011) Nature 474, 654-7. 3 . Hrecka, K. et al. (2011) Nature 474, 658-61. 4 . Powell, R.D. et al. (2011) J Biol Chem 286, 43596-600. 5 . Goldstone, D.C. et al. (2011) Nature 480, 379-82. 6 . Lahouassa, H. et al. (2012) Nat Immunol 13, 223-8. |
Flow cytometric analysis of THP-1 cells, untreated (green) or treated with TPA #4174 (80 nM, 16 hr; red), using Phospho-SAMHD1 (Thr592) (D7O2M) Rabbit mAb (PE Conjugate) (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742 (dashed lines). | |
Flow cytometric analysis of Jurkat cells (blue) and THP-1 cells (green) using Phospho-SAMHD1 (Thr592) (D7O2M) Rabbit mAb (PE Conjugate) (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742 (dashed lines). |
