| 货号 | 59056S |
| 同种亚型 | Rabbit |
| 反应种属 | Human, Mouse, Rat, Monkey |
| 应用 | F |
| 目标/特异性 | Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb (PE Conjugate) detects endogenous levels of SEK1/MKK4 phosphorylated at Ser257. |
| 使用方法 | Flow Cytometry (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5). |
| 存放说明 | 4C |
| 参考文献 | 1 . Davis, R.J. (1994) Trends Biochem. Sci. 19, 470-473. 2 . Sanchez, I. et al. (1994) Nature 372, 794-798. 3 . Yan, M. et al. (1994) Nature 372, 798-800. 4 . Kyriakis, J.M. et al. (1994) Nature 369, 156-160. 5 . Park, H. et al. (2002) J. Biol. Chem. 277, 2573-2578. |
Flow Cytometric analysis of 293T cells, untreated (blue) or UV-treated (green), using Phospho-SEK1/MKK4(Ser257)(C36C11) Rabbit mAb (PE Conjugate). |
