| 货号 | 52821S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | IF,F |
| 目标/特异性 | Phospho-CD79A (Tyr182) (D1B9) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of human CD79A protein only when phosphorylated on Tyr188. This corresponds to Tyr182 of mouse CD79A protein. |
| 使用方法 | Immunofluorescence (Immunocytochemistry) (1:50) Flow Cytometry (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Antigen receptors found on the surface of B cells contain a heterodimeric signaling component composed of CD79A and CD79B, also known as Ig α and Ig β, respectively (1,2). Presence of this receptor complex is essential for B-cell development and function (3). Together these two proteins and the associated B cell receptor initiate intracellular signaling following antigen binding (4,5). An immunoreceptor tyrosine-based activation motif (ITAM) found in the CD79A intracellular region appears to be important for its function (6). Antigen binding precedes formation of the CD79A and CD79B heterodimer and subsequent activation of receptor associated kinases (7). Research has shown that CD79A is a marker for B-lineage lymphoblastic leukemia (8). Additionally, investigators have found that mutations in the CD79A(MB1) gene are associated with abnormally low levels of functional B cell receptors in some cases of chronic B cell lymphocytic leukemia (9). |
| 存放说明 | 4C |
| 参考文献 | 1 . van Noesel, C.J. et al. (1991) J Immunol 146, 3881-8. 2 . Minegishi, Y. et al. (1999) J Clin Invest 104, 1115-21. 3 . Yu, L.M. and Chang, T.W. (1992) J Immunol 148, 633-7. 4 . Storch, B. et al. (2007) Eur J Immunol 37, 252-60. 5 . Mason, D.Y. et al. (1995) Blood 86, 1453-9. 6 . Luisiri, P. et al. (1996) J Biol Chem 271, 5158-63. 7 . Pike, K.A. et al. (2004) J Immunol 172, 2210-8. 8 . Astsaturov, I.A. et al. (1996) Leukemia 10, 769-73. 9 . Vuillier, F. et al. (2005) Blood 105, 2933-40. |
Flow cytometric analysis of Ramos cells, untreated (blue) or treated with anti-human IgM (12 μg/ml, 10 min; green), using Phospho-CD79A (Tyr182) (D1B9) Rabbit mAb (Alexa Fluor® 488 Conjugate). |
