4E-BP1 (53H11) Rabbit mAb (Biotinylated)
货号：
8594S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 8594S |
| 描述 | This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated 4E-BP1 (53H11) Rabbit mAb #9644. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | 4E-BP1 (53H11) Rabbit mAb detects endogenous levels of total 4E-BP1 protein. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 标记 | Biotin |
| 背景 | Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). |
| 存放说明 | -20C |
| 计算分子量 | 15-20 |
参考图片Western blot analysis of extracts from MCF7 and HeLa cells using 4E-BP1 (53H11) Rabbit mAb (Biotinylated). 使用4E-BP1 (53H11) Rabbit mAb兔单抗(生物素标记的)，Western Blot分析MCF7和HeLa细胞中4E-BP1蛋白水平。 |
