ALK (C26G7) Rabbit mAb (Sepharose® Bead Conjugate)
货号：
5611S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 5611S |
| 描述 | This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. ALK (C26G7) Rabbit mAb (Sepharose Bead Conjugate) is useful for immunoprecipitation assays. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated ALK (C26G7) Rabbit mAb #3333. |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | IP |
| 使用方法 | IP(1:20) |
性能| 供应商 | CST |
| 标记 | Sepharose Bead |
| 背景 | Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ, and PI3 kinase (1). ALK was originally discovered as an NPM (nucleophosmin)-ALK fusion protein produced by a translocation (4). The NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Activation of PLCγ by NPM-ALK has been suggested to be a crucial step for its mitogenic activity and may be important in the pathogenesis of anaplastic lymphomas (5). A distinct ALK oncogenic fusion protein involving ALK and EML4 has been described from a non-small cell lung cancer cell line, with corresponding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase domain of ALK (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 80 (NPM-ALK), 220 (ALK) |
参考图片Immunoprecipitation of Karpas-299 cell lysates using Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423 and ALK (C26G7) Rabbit mAb (Sepharose Bead Conjugate). The western blot was probed using ALK (31F12) Mouse mAb #3791. 用结合结合琼脂糖凝胶的抗兔源单克隆抗体IgG XP®同型对照#3423和结合结合琼脂糖凝胶的抗ALK的兔源单克隆抗体(C26G7) #3333对Karpas-299细胞的裂解液进行免疫沉淀。蛋白质印记检测使用抗ALK的小鼠源单克隆抗体(31F12) #3791。 |
