AMPKα (23A3) Rabbit mAb (Sepharose®Bead Conjugate)
货号：
6707S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 6707S |
| 描述 | This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. AMPKα (23A3) Rabbit mAb (Sepharose Bead Conjugate) is useful for immunoprecipitation assays. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated AMPKα (23A3) Rabbit mAb #2603. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | IP |
| 目标/特异性 | AMPKα (23A3) Rabbit mAb (Sepharose Bead Conjugate) detects endogenous levels of total AMPKα protein. |
| 使用方法 | IP(1:20) |
性能| 供应商 | CST |
| 标记 | Sepharose Bead |
| 背景 | AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, 2; β1, 2; γ1, 2, 3) (2). The kinase is activated by an elevated AMP/ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia, and ischemia (1). The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPKα at Thr172 in the activation loop, and this phosphorylation is required for AMPK activation (3-5). AMPKα is also phosphorylated at Thr258 and Ser485 (for α1; Ser491 for α2). The upstream kinase and the biological significance of these phosphorylation events have yet to be elucidated (6). The β1 subunit is post-translationally modified by myristoylation and multi-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108, and Ser182 (6,7). Phosphorylation at Ser108 of the β1 subunit seems to be required for the activation of AMPK enzyme, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization (7). Several mutations in AMPKγ subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumulation in heart or skeletal muscle (1,2). Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS (1). |
| 存放说明 | -20C |
| 计算分子量 | 62 |
参考图片Immunoprecipitation of 293 and C6 cell lysates using Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423 and AMPKα (23A3) Rabbit mAb (Sepharose Bead Conjugate). The western blot was probed using AMPKα (F6) Mouse mAb #2793. 免疫沉淀检测293和C6细胞提取物，所用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423和AMPKα (23A3) Rabbit mAb兔单抗 (Sepharose Bead Conjugate)。Western实验所用抗体为AMPKα (F6) Mouse mAb鼠单抗 #2793 |
