Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Biotinylated)
货号：
9654S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 9654S |
| 描述 | This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Biotinylated) detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full-length caspase-3 or other cleaved caspases. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 标记 | Biotin |
| 背景 | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2). |
| 存放说明 | -20C |
| 计算分子量 | 17, 19 |
参考图片Western blot analysis of extracts from HeLa cells, untreated or treated with Staurosporine #9953 (1 μM, 3 hrs), using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Biotinylated) and detected with Streptavidin-HRP #3999. 采用 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (生物素标记)兔单抗对处理或 Staurosporine #9953 (1 μM, 3 hrs)处理的 HeLa 细胞进行Western blot 检测，用 Streptavidin-HRP #3999 进行检测。 |
