Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 594 Conjugate)
货号：
8172S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 8172S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb #9579. |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | IF-IC |
| 目标/特异性 | Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 594 Conjugate) recognizes endogenous levels of caspase-3 protein only when cleaved at Asp175. |
| 使用方法 | IF-IC(1:50) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 594 |
| 背景 | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2). |
| 存放说明 | 4C |
参考图片Immunofluorescent analysis of HeLa cells, untreated (left) or treated with Staurosporine #9953 (1 μM, 4 hr; right), using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 594 Conjugate) (red). Actin filaments were labeled with Alexa Fluor® 488 phalloidin #8878 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 免疫荧光分析HeLa细胞，未处理（左）和 Staurosporine #9953 （1 μM, 4 hr；右）处理，使用抗体为Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb 兔单抗 (Alexa Fluor® 594 Conjugate)（红色）。Alexa Fluor® 488 phalloidin #8878（绿色）标记肌动蛋白丝。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 |
