E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 594 Conjugate)
货号：
7687S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 7687S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated E-Cadherin (24E10) Rabbit mAb #3195. |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | IF-IC |
| 目标/特异性 | E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 594 Conjugate) recognizes endogenous levels of total E-cadherin protein. The antibody does not cross-react with related family members, such as N-cadherin. |
| 使用方法 | IF-IC(1:50) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 594 |
| 背景 | Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). |
| 存放说明 | 4C |
参考图片Immunofluorescent analysis of MCF7 (left) and HeLa (right) cells using E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 594 Conjugate) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).免疫荧光分析 MCF7 (左图)和HeLa (右图)细胞，红色为E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 594 Conjugate) ，蓝色伪彩 = DRAQ5® #4084 (DNA荧光染料)。 |
