| 货号 | 3368S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in cells transfected with GST-tagged protein. |
| 反应种属 | All |
| 来源宿主 | Mouse |
| 应用 | IF-IC/F |
| 使用方法 | F(1:50) IF-IC (1:50) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties. |
| 存放说明 | 4C |
Confocal immunofluorescent analysis of COS cells, mock transfected (left) or transfected with a GST-tagged protein (right), using GST (26H1) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).荧光共聚焦免疫荧光分析COS细胞，上图为mock转染组，下图为GST标签蛋白转染组。所用抗体为GST (26H1) Mouse mAb (Alexa Fluor ® 488 Conjugate) (绿色)。肌动蛋白丝被DY-554 phalloidin (红色)标记。蓝色伪彩为荧光染料，信息为DRAQ5 ® #4084。 | |
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with GST (green), using GST (26H1) Mouse mAb (Alexa Fluor® 488 Conjugate.)流式细胞术分析COS细胞，蓝色为未转染组，绿色为GST转染组，所用抗体为GST (26H1) Mouse mAb (Alexa Fluor ® 488Conjugate)。 |
