| 货号 | 2350S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofuorescent analysis in cells transfected with HA-tagged protein. |
| 来源宿主 | Mouse |
| 应用 | IF-IC/F |
| 使用方法 | F(1:50) IF-IC (1:800) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties. |
| 存放说明 | 4C |
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with HA-tagged Akt3 (green), using HA-Tag (6E2) Mouse mAb (Alexa Fluor® 488 Conjugate).流式细胞术分析COS细胞，蓝色为未转染组，绿色为HA-tagged Akt3 转染组，所用抗体为HA-Tag (6E2) Mouse mAb (Alexa Fluor ® 488 Conjugate)。 | |
Confocal immunofluorescent analysis of COS cells, mock-transfected (left) or transfected with HA-tagged protein (right), using HA-Tag (6E2) Mouse mAb (Alexa Fluor® 488 Conjugate) #2350. Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).激光共聚焦显微镜分析COS细胞，左图为mock未转染组，右图为HA-Tag转染组，所用抗体为HA-Tag (6E2) Mouse mAb (Alexa Fluor ® 488 Conjugate) #2350。肌动蛋白丝采用 DY-554 phalloidin（红色）。蓝色伪彩为荧光DNA燃料，产品信息为DRAQ5 ® #4084 。 |
