| 货号 | 3444S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in cells transfected with HA-tagged protein.CST公司的 |
| 来源宿主 | Mouse |
| 应用 | IF-IC/F |
| 使用方法 | F(1:50) IF-IC (1:100) |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties. |
| 存放说明 | 4C |
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with HA-tagged DLL1 (green), using HA-Tag (6E2) Mouse mAb (Alexa Fluor® 647 Conjugate).流式细胞术分析COS细胞，蓝色为未转染组，绿色为HA-tagged DLL1转染组，所用抗体为HA-Tag (6E2) Mouse mAb (Alexa Fluor ® 647 Conjugate) 。 | |
Confocal immunofluorescent analysis of COS cells, untransfected (left) or transfected with an HA-tagged protein (right), using HA-Tag (6E2) Mouse mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor) and Di-Methyl-Histone H3 (Lys27) Antibody #9755 (green). Actin filaments were labeled with DY-554 phalloidin (red).激光共聚焦免疫荧光分析COS细胞，左图为未转染组，右图为采用含HA标签蛋白转染组，所用抗体为HA-Tag (6E2) Mouse mAb (Alexa Fluor ® 647 Conjugate) (蓝色伪彩) 和Di-Methyl-Histone H3 (Lys27) Antibody #9755 (绿色)。肌动蛋白丝采用DY-554 phalloidin (红色). |
