货号 | 5017S |
描述 | This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions and tested using COS cells transfected with HA-tagged protein. |
反应种属 | All |
来源宿主 | Rabbit |
应用 | W/F |
使用方法 | WB(1:1000) F (1:400) |
供应商 | CST |
标记 | Biotin |
背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties. |
存放说明 | -20C |
Western blot analysis of extracts from COS cells, untransfected or transfected with a construct overexpressing HA-Akt, using HA-Tag (C29F4) Rabbit mAb (Biotinylated). Detection was performed with Streptavidin-HRP followed by LumiGlo® and Peroxidase #7003.Western blot分析COS-7细胞提取物,未转染组和过表达HA-Akt的转染组,所用抗体为HA-Tag (C29F4) Rabbit mAb (生物素化) 。 | |
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with HA-Tag (green), using HA-Tag (C29F4) Rabbit mAb (Biotinylated).流式细胞术分析COS细胞,蓝色是未转染组,绿色是用HA标签转染组,苏勇抗体为HA-Tag (C29F4) Rabbit mAb (生物素化)。 |