货号 | 2279S |
描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in cells transfected with Myc-tagged protein. |
反应种属 | All |
来源宿主 | Mouse |
应用 | IF-IC/F |
使用方法 | F(1:50) IF-IC (1:400) |
供应商 | CST |
标记 | Alexa Fluor 488 |
背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties. |
存放说明 | 4C |
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with Myc-tagged Akt (green), using Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 488 Conjugate).流式细胞术分选COS细胞,蓝色为未转染组,绿色为Myc-tagged Akt 转染的细胞,所用抗体为using Myc-Tag (9B11) Mouse mAb (Alexa Fluor ® 488 Conjugate)。 | |
Confocal immunofluorescent analysis of parental COS cells (left) and COS cells expressing a Myc-tagged protein (right), using Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光分析母COS细胞(左图),右图为Myc-tagged Akt 转染的细胞,所用抗体为Myc-Tag (9B11) Mouse mAb (Alexa Fluor ® 488 Conjugate) (绿色)。蓝色伪彩为荧光DNA燃料,产品信息为DRAQ5 ® #4084 。 |