| 货号 | 2233S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in cells transfected with Myc-tagged protein. |
| 反应种属 | All |
| 来源宿主 | Mouse |
| 应用 | IF-IC/F |
| 使用方法 | F(1:50) IF-IC (1:100) |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties. |
| 存放说明 | 4C |
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with Myc-tagged Akt (green), using Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate).流式细胞术分析COS细胞，蓝色为未转染组，绿色为Myc-tagged Akt转染的细胞，所用抗体为Myc-Tag (9B11) Mouse mAb (Alexa Fluor ® 647 Conjugate)。 | |
Confocal immunofluorescent analysis of COS cells expressing a Myc-tagged protein (left) and untreated (right), using Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Actin filaments have been labeled with DY-554 phalloidin (red).激光共聚焦免疫荧光分析表达Myc标签蛋白转染COS细胞（上图）和未转染组（下图），所用抗体为Myc-Tag (9B11) Mouse mAb (Alexa Fluor ® 647 Conjugate) (蓝色伪彩)，肌动蛋白丝采用DY-554 phalloidin (红色）标记。 |
