Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor® 488 Conjugate)
货号：
8024S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 8024S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Neurofilament-L (C28E10) Rabbit mAb #2837. |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | IF-F |
| 目标/特异性 | Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total Neurofilament-L protein. |
| 使用方法 | IF-F(1:50) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Neurofilament accumulations are found in many human neurological disorders including Parkinsons disease (in Lewy bodies along with α-synuclein), Alzheimers disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1). |
| 存放说明 | 4C |
参考图片Confocal immunofluorescent analysis of normal rat cerebellum using Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).对正常大鼠小脑使用Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor? 488 Conjugate)（绿色）和GFAP (GA5) Mouse mAb #3670（红色）进行共聚焦免疫荧光分析。蓝色伪彩=DRAQ5 TM#4084(荧光DNA染料)。 |
