Pan-Actin (D18C11) Rabbit mAb (HRP Conjugate)
货号：
12748S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 12748S |
| 描述 | This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Pan-Actin (D18C11) Rabbit mAb #8456. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Pan-Actin (D18C11) Rabbit mAb (HRP Conjugate) recognizes endogenous levels of total actin protein (all isoforms). |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 标记 | HRP |
| 背景 | Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (6). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
参考图片Western blot analysis of extracts from various cell lines using Pan-Actin (D18C11) Rabbit mAb (HRP Conjugate).使用Pan-Actin (D18C11) Rabbit mAb（偶联HRP）对多种细胞提取物进行western blot分析。 |
