| 货号 | 6704S |
| 描述 | This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. PARP (46D11) Rabbit mAb (Sepharose Bead Conjugate) is useful for immunoprecipitation assays. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated PARP (46D11) Rabbit mAb #9532. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | IP |
| 使用方法 | IP(1:20) |
| 供应商 | CST |
| 标记 | Sepharose Bead |
| 背景 | PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). |
| 存放说明 | -20C |
| 计算分子量 | 116, 89 |
Immunoprecipitation of THP-1 cell lysates, untreated or cycloheximide and TNFα-treated, using PARP (46D11) Rabbit mAb (Sepharose Bead Conjugate). The blot was probed using PARP (46D11) Rabbit mAb #9532. 免疫沉淀分析Jurkat细胞裂解液，包括未处理或放线菌素和TNFα处理，使用抗体为PARP (46D11) Rabbit mAb (Sepharose Bead Conjugate)兔单抗。印迹检测用的抗体的是PARP (46D11) Rabbit mAb兔单抗#9532。 | |
Immunoprecipitation of HeLa cell lysates, untreated or staurosporine-treated, using PARP (46D11) Rabbit mAb (Sepharose Bead Conjugate). The blot was probed using PARP (46D11) Rabbit mAb #9532. 免疫沉淀分析HeLa 细胞裂解液，未处理或星形孢菌素处理，使用抗体为PARP (46D11) Rabbit mAb (Sepharose Bead Conjugate)兔单抗。印迹检测所用的抗体是PARP (46D11) Rabbit mAb兔单抗#9532。 |
