PDI (C81H6) Rabbit mAb (Alexa Fluor® 488 Conjugate)
货号：
5051S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 5051S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated PDI (C81H6) Rabbit mAb #3501. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | IF-IC |
| 目标/特异性 | PDI (C81H6) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total PDI protein. |
| 使用方法 | IF-IC(1:50) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | During their synthesis, secretory proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes the ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation in folding proteins (3). |
| 存放说明 | 4C |
参考图片Immunofluorescent analysis of NIH/3T3 cells using PDI (C81H6) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) and a β-Actin Mouse mAb (Alexa Fluor® 594 Conjugate) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对NIH/3T3细胞使用PDI (C81H6) Rabbit mAb(Alexa Fluor® 488 Conjugate)（绿）和β-Actin Mouse mAb (Alexa Fluor® 594 Conjugate) （红）进行免疫荧光分析。蓝色伪彩=DRAQ5®(荧光DNA染料)。 |
