Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated)
货号：
5102S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 5102S |
| 反应种属 | Human/Mouse/Rat/Hamster |
| 来源宿主 | Mouse |
| 应用 | IP |
| 目标/特异性 | Phospho-Akt (Ser473) (587F11) Mouse mAb detects endogenous levels of Akt only when phosphorylated at Ser473. This antibody does not detect Akt phosphorylated at other sites or related kinases such as PKC and p70 S6 kinase. This is a biotinylated version of the #4051 antibody and is expected to continue to recognize other species such rat and hamster. |
| 使用方法 | IP(1:50) |
性能| 供应商 | CST |
| 标记 | Biotin |
| 背景 | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
参考图片Akt phosphorylated at serine 473 was immunoprecipitated from untreated or LY294002 treated Jurkat cell extracts, using Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated). This immunocomplex was then pulled down using streptavidin beads. Western blotting was performed with Phospho-Akt (Ser473) Antibody #9271. Ser473位点磷酸化的Akt从未处理和LY294002处理的Jurkat 细胞提取物中免疫沉淀下来，所用抗体为Phospho-Akt (Ser473) (587F11) Mouse mAb鼠单抗 (Biotinylated)。免疫复合物采用链霉素亲和素微珠拉下来。采用Phospho-Akt (Ser473) Antibody #9271进行western blot。 |
