Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (HRP Conjugate)
货号：
6950S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 6950S |
| 描述 | This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb #9614. |
| 反应种属 | Mouse/D.melanogaster/All |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (HRP Conjugate) recognizes peptides and proteins containing phospho-serine/threonine preceded by arginine at the -3 position. There is some preference observed for peptides that contain phospho-serine/threonine preceded by arginine at both positions -5 and -3. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 标记 | HRP |
| 背景 | An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from CST are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery. |
| 存放说明 | -20C |
参考图片Western blot analysis of extracts from Jurkat and HeLa cells, untreated or treated with Calyculin A #9902 (0.1 µM, 45 min), using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (HRP Conjugate). 对未处理或0.1uM Calyculin A #9902裂解前处理45分钟的Jurkat和HeLa细胞抽提液使用Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (HRP Conjugate)进行Western blot分析。 |
