Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Magnetic Bead Conjugate)
货号：
8050S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 8050S |
| 描述 | This Cell Signaling Technology antibody is immobilized by the covalent reaction of hydrazinonicotinamide-modifed antibody with formylbenzamide-modified magnetic bead. Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Magnetic Bead Conjugate) is useful for immunoprecipitation of phosphorylated Akt substrate proteins. |
| 反应种属 | Mouse/D.melanogaster/All |
| 来源宿主 | Rabbit |
| 应用 | IP |
| 目标/特异性 | Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Magnetic Bead Conjugate) recognizes peptides and proteins containing phospho-Ser/Thr preceded by Arg at the -3 position. There is some preference observed for peptides that contain phospho-Ser/Thr preceded by Arg at both positions -5 and -3. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | IP(1:20) |
性能| 供应商 | CST |
| 标记 | Magnetic Bead |
| 背景 | An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from CST are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery. |
| 存放说明 | 4C |
参考图片Immunoprecipitation of Jurkat cell lysates, untreated or treated with Calyculin A, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Magnetic Bead Conjugate) (lane 1 and 2) and Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) #8726 (lane 3 and 4). The western blot was probed using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (HRP Conjugate) #6950. 对Jurkat细胞抽提物，未处理或Calyculin A处理，使用Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Magnetic Bead Conjugate)（列1和列2）或Rabbit (DA1E) mAb IgG XP庐 Isotype Control (Magnetic Bead Conjugate) #8726（列3和列4）进行免疫共沉淀。Western blot使用Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (HRP Conjugate) #6950。 |
