Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Sepharose®Bead Conjugate)
货号：
9646S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 9646S |
| 描述 | This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of phosphorylated Akt substrate proteins. |
| 反应种属 | Mouse/D.melanogaster/All |
| 来源宿主 | Rabbit |
| 应用 | IP |
| 目标/特异性 | Phospho-(Ser/Thr) Akt Substrate Motif (RXXS*/T*) (110B7) Rabbit mAb (Sepharose Bead Conjugate) recognizes peptides and proteins containing phospho-serine/threonine preceded by arginine at the -3 position. There is some preference observed for peptides that contain phospho-serine/threonine preceded by arginine at both positions -5 and -3. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | IP(1:20) |
性能| 供应商 | CST |
| 标记 | Sepharose Bead |
| 背景 | An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from CST are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery. |
| 存放说明 | -20C |
参考图片Immunoprecipitation of NIH/3T3 cell lysates, untreated or PDGF-treated, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Sepharose Bead Conjugate) compared to supernatants collected after immunoprecipitation from the same lysate. The western blot was probed using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb #9614. NIH/3T3裂解液，未处理或PDGF处理，使用Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Sepharose Bead Conjugate)进行免疫共沉淀分析，并与共沉淀后裂解液的上清液进行对照。Western blot使用Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb #9614。 |
