Phospho-Bad (Ser112) (7E11) Mouse mAb (Biotinylated)
货号：
5193S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 5193S |
| 描述 | This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Bad (Ser112) (7E11) Mouse mAb #9296. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W |
| 目标/特异性 | Phospho-Bad (Ser112) (7E11) Mouse mAb (Biotinylated) recognizes endogenous levels of Bad only when phosphorylated at Ser112. The Ser112 nomenclature is based upon the mouse sequence. The analogous phosphorylation site is Ser75 in human and Ser113 in rat. This antibody does not detect Bad phosphorylated at other sites, nor does it detect related family members. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 标记 | Biotin |
| 背景 | Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
参考图片Western blot analysis of extracts from OVCAR8 cells, untreated (-) or λ phosphatase-treated (+), using Phospho-Bad (Ser112) (7E11) Mouse mAb (Biotinylated). Western blot 检测OVCAR8细胞提取物，未处理 (-)或λ磷酸酶处理 (+)，使用抗体为Phospho-Bad (Ser112) (7E11) Mouse mAb (Biotinylated)鼠单抗。 |
