Phospho-IκBα (Ser32) (14D4) Rabbit mAb (Biotinylated)
货号：
5209S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 5209S |
| 描述 | This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-IκBα (Ser32) (14D4) Rabbit mAb #2859. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-IκBα (Ser32) (14D4) Rabbit mAb (Biotinylated) detects endogenous levels of IκBα only when phosphorylated at Ser32. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 标记 | Biotin |
| 背景 | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
参考图片Western blot analysis of extracts from HeLa cells, untreated or treated with human TNF-α (#8902, 20 ng/ml) for 5 minutes, using Phospho-IκBα (Ser32) (14D4) Rabbit mAb (Biotinylated).Western免疫印迹。用Phospho-IκBα (Ser32) (14D4) Rabbit mAb (Biotinylated)研究未经处理的和经 TNF-α #8902 20 ng/ml,for 5 minutes 处理的HeLa 细胞的细胞提取液。 |
