| 货号 | 3958S |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 标记 | Biotin |
| 背景 | MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII. |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Western blot analysis of extracts of HeLa cells, untreated or TPA-treated, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) and Streptavidin-HRP (#3999). | |
western blot方法检测细胞提取物：未处理和TPA处理的HeLa细胞，使用的抗体是Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated)兔单抗和 Streptavidin-HRP (#3999)。 |
