Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated)
货号：
4025S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 4025S |
| 描述 | This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 reacts with human, mouse, rat, monkey, hamster, and pig phospho-NF-κB. CST expects that Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated) will also recognize phospho-NF-κB in these species. |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/Pig |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated) detects NF-κB p65 protein only when phosphorylated at Ser536. It does not cross-react with the p50 subunit or other related proteins. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 标记 | Biotin |
| 背景 | Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11). |
| 存放说明 | -20C |
| 计算分子量 | 65 |
参考图片Western blot analysis of NF-κB Control Cell Extracts #9243 (HeLa untreated or treated with hTNF-α #8902, 20 ng/ml, 5 min.), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated).Western免疫印迹。用Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated)研究NF-κB Control Cell Extracts #9243 (未经处理的和经hTNF-α #8902, 20 ng/ml, 5 min处理的HeLa )。 |
