Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (Sepharose®Bead Conjugate)
货号：
9219S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 9219S |
| 反应种属 | Human/Mouse/Rat/Monkey/S.cerevisiae |
| 来源宿主 | Mouse |
| 应用 | IP |
| 使用方法 | IP(1:20) |
性能| 供应商 | CST |
| 标记 | Sepharose Bead |
| 背景 | p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).
SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
参考图片p38 MAPK activity of UV-treated or untreated C6 cell extracts was analyzed by IP/kinase assay. Cell extracts were immunoprecipitated with Phospho-p38 MAPK (Thr180/Tyr182) Mouse mAb (Sepharose Bead Conjugate). In vitro kinase assays were performed using ATF-2 Fusion Protein #9224 as a substrate. Phosphorylation of ATF-2 at Thr71 was measured by Western blot using Phospho-ATF-2 (Thr71) Antibody.IP/kinase分析UV处理和未经处理的C6细胞提取物中p38 MAPK的活性。细胞提取物经免疫沉淀分析，使用的抗体是Phospho-p38 MAPK (Thr180/Tyr182) Mouse mAb (Sepharose Bead Conjugate).。体外分析使用ATF-2 Fusion Protein #9224作为底物。Western blot分析检测ATF-2蛋白苏氨酸（71位点）的磷酸化，使用的抗体是Phospho-ATF-2 (Thr71) Antibody。 |
