Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (Sepharose®Bead Conjugate)
货号：
4306S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 4306S |
| 反应种属 | Human/Mouse/Rat/D.melanogaster/S.cerevisiae |
| 来源宿主 | Rabbit |
| 应用 | IP |
| 使用方法 | IP(1:20) |
性能| 供应商 | CST |
| 标记 | Sepharose Bead |
| 背景 | The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5). |
| 存放说明 | -20C |
| 计算分子量 | 46, 54 |
参考图片Immunoprecipitation of C6 cell lysates, untreated or anisomycin-treated, using Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423 and Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (Sepharose Bead Conjugate). The western blot was probed using Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb #9255.免疫沉淀方法检测细胞提取物：未处理和茴香霉素处理的C6 细胞，使用的抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423和Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (Sepharose Bead Conjugate).免疫印迹试验中使用的抗体是Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb #9255。 |
