Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb (Biotinylated)
货号：
9576S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 9576S |
| 描述 | This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516. |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb (Biotinylated) detects endogenous levels of Smad1/5 only when dually phosphorylated at Ser463 and Ser465. The antibody does not cross-react with other Smad-related proteins. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 标记 | Biotin |
| 背景 | Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
参考图片Western blot analysis of extracts from untreated or BMP4-treated HT-1080 cells using Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb (Biotinylated) and detected using Streptavidin-HRP #3999. 使用Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb兔单抗(生物素标记) 对未处理或经过BMP￥处理的HT-1080细胞进行western blot分析， 使用Streptavidin-HRP进行检测 #3999. |
