| 货号 | 5465S |
| 描述 | This Cell Signaling Technology® antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411. |
| 反应种属 | Human/Mouse/Rat/All |
| 来源宿主 | Mouse |
| 应用 | W/E-P |
| 目标/特异性 | Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate) is a high affinity antibody. ELISAs against a wide variety of phosphopeptides indicate that P-Tyr-100 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence. 2D gel western blot analysis of pervanadate-treated cell extracts also shows that P-Tyr-100 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-100 does not cross-react with peptides containing phospho-Ser or phospho-Thr. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:2000) E-P (1:1000) |
| 供应商 | CST |
| 标记 | HRP |
| 背景 | Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. |
| 存放说明 | -20C |
Western blot analysis of extracts from Jurkat and 3T3/NIH cells, untreated and treated with sodium vanadate (1 mM for 30 minutes), using Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate). 对NIH/3T3细胞系抽提物，未处理或钒酸钠（1mM，30分钟）处理，使用Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate)进行Western blot分析。 | |
Signal-to-noise (S/N) values of phosphopeptide versus nonphosphopeptide signals using LumiGLO® and Peroxide Reagents (#7003). 使用LumiGLO® and Peroxide Reagents #7003的磷酸化肽段和非磷酸化肽段的信噪比。 |
