Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Magnetic Bead Conjugate)
货号：
8095S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 8095S |
| 描述 | This Cell Signaling Technology (CST) antibody is immobilized by the covalent reaction of hydrazinonicotinamide-modifed antibody with formylbenzamide-modified magnetic bead. Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Magnetic Bead Conjugate) is useful for immunoprecipitation assays. The unconjugated Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 reacts with all species of phospho-tyrosine protein. CST expects that Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Magnetic Bead Conjugate) will also recognize phospho proteins in all species. |
| 反应种属 | Human/Mouse/Rat/All |
| 来源宿主 | Mouse |
| 应用 | IP |
| 目标/特异性 | Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Magnetic Bead Conjugate) contains high affinity beads. ELISAs against a wide variety of phosphopeptides indicate that P-Tyr-100 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence. 2D gel western blot analysis of pervanadate-treated cell extracts shows that P-Tyr-100 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-100 does not cross-react with peptides containing phospho-Ser or phospho-Thr. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | IP(1:20) |
性能| 供应商 | CST |
| 标记 | Magnetic Bead |
| 背景 | Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. |
| 存放说明 | 4C |
参考图片Immunoprecipitation of Jurkat cell extracts, untreated or pervanadate-treated, using Mouse IgG (Magnetic Bead Conjugate) #5873 (lanes 1 and 2) or Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Magnetic Bead Conjugate) (lanes 3 and 4). The western blot was probed using Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate) #5465. 对Jurkat细胞抽提物，未处理或过钒酸盐处理，使用Mouse IgG (Magnetic Bead Conjugate) #5873（列1和列2）或Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Magnetic Bead Conjugate)（列3和列4）进行免疫共沉淀。Western Blot探针为Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate) #5465。 |
