Cas9 (7A9-3A3) Mouse mAb (Alexa Fluor® 488 Conjugate)【科瑞奥博】

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Cas9 (7A9-3A3) Mouse mAb (Alexa Fluor® 488 Conjugate)

货号： 34963S 基本售价： 4060.0 元规格： -

产品信息

概述
货号	34963S
同种亚型	Mouse IgG1
反应种属	All
来源宿主	Mouse IgG1
应用	IF-IC F
目标/特异性	Cas9 (7A9-3A3) Mouse mAb (Alexa Fluor^® 488 Conjugate) recognizes transfected levels of total Cas9 protein.
使用方法	F(1:50) IF-IC (1:100)

性能
供应商	CST
灵敏度	Transfected Only
背景
存放说明	4C
参考文献	1 . Horvath, P. and Barrangou, R. (2010) Science 327, 167-70. 2 . Wiedenheft, B. et al. (2012) Nature 482, 331-8. 3 . Singh, P. et al. (2015) Genetics 199, 1-15. 4 . Cong, L. et al. (2013) Science 339, 819-23. 5 . Mali, P. et al. (2013) Science 339, 823-6. 6 . Li, D. et al. (2013) Nat Biotechnol 31, 681-3. 7 . Shen, B. et al. (2013) Cell Res 23, 720-3. 8 . Niu, Y. et al. (2014) Cell 156, 836-43.

参考图片

Flow cytometric analysis of HEK-293 cells untreated (blue) or transfected with NPRL2 CRISPR/Cas9 KO plasmid (green) using

Confocal immunofluorescent analysis of Cas9 expression in NPRL2 CRISPR/Cas9 KO plasmid transfected (left) and untransfected (right) HEK-293 cells using Cas9 (7A9-3A3) Mouse mAb (Alexa Fluor^® 488 Conjugate) (green). Cas9 expressing cells were generated by transient transfection of Cas9 and NPRL2-specific guide sequences. Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye). HEK-293/NPRL2 knockout cells were kindly provided by Rachel Wolfson, Lynne Chantranupong, and David Sabatini from MIT, Cambridge, MA.