Cas9 (7A9-3A3) Mouse mAb (Alexa Fluor® 647 Conjugate)【科瑞奥博】

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Cas9 (7A9-3A3) Mouse mAb (Alexa Fluor® 647 Conjugate)

货号： 48796S 基本售价： 4060.0 元规格： -

产品信息

概述
货号	48796S
同种亚型	Mouse IgG1
反应种属	All
来源宿主	Mouse IgG1
应用	IF-IC F
目标/特异性	Cas9 (7A9-3A3) Mouse mAb (Alexa Fluor^® 647 Conjugate) recognizes transfected levels of total Cas9 protein.
使用方法	F(1:50) IF-IC (1:400)

性能
供应商	CST
灵敏度	Transfected Only
背景
存放说明	4C
参考文献	1 . Horvath, P. and Barrangou, R. (2010) Science 327, 167-70. 2 . Wiedenheft, B. et al. (2012) Nature 482, 331-8. 3 . Singh, P. et al. (2015) Genetics 199, 1-15. 4 . Cong, L. et al. (2013) Science 339, 819-23. 5 . Mali, P. et al. (2013) Science 339, 823-6. 6 . Li, D. et al. (2013) Nat Biotechnol 31, 681-3. 7 . Shen, B. et al. (2013) Cell Res 23, 720-3. 8 . Niu, Y. et al. (2014) Cell 156, 836-43.

参考图片

Confocal immunofluorescent analysis of Cas9 expression in Nprl2-deficient (left) and untreated (right) HEK-293 cells using Cas9 (7A9-3A3) Mouse mAb (Alex Fluor^® 647 Conjugate) (red). Actin filaments were labeled with DyLight™ 488 Phalloidin #12935 (green). Blue pseudocolor = Propidium Iodide (PI)/RNase Staining Solution #4087. Nprl2 expression was knocked out by transient transfection of Cas9 and Nprl2-specific guide sequences. HEK-293/Nprl2 -/- cells were kindly provided by Rachel Wolfson, Lynne Chantranupong, and David Sabatini of MIT, Cambridge, MA.

Flow cytometric analysis of Cas9 expression in untreated (blue) or Nprl2-deficient (green) HEK-293 cells using Cas9 (7A9-3A3) Mouse mAb (Alex Fluor^® 647 Conjugate). Nprl2 expression was knocked out by transient transfection of Cas9 and Nprl2-specific guide sequences. HEK-293/Nprl2 -/- cells were kindly provided by Rachel Wolfson, Lynne Chantranupong, and David Sabatini of MIT, Cambridge, MA.