Rictor (D16H9) Rabbit mAb (Sepharose® Bead Conjugate)
货号：
5379S 基本售价：
4060.0 元 规格：
-
产品信息
概述| 货号 | 5379S |
| 描述 | This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Rictor (D16H9) Rabbit mAb (Sepharose Bead Conjugate) is useful for immunoprecipitation assays. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Rictor (D16H9) Rabbit mAb #9476. |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | IP |
| 目标/特异性 | Rictor (D16H9) Rabbit mAb (Sepharose Bead Conjugate) detects endogenous levels of total rictor protein. |
| 使用方法 | IP(1:20) |
性能| 供应商 | CST |
| 标记 | Sepharose Bead |
| 背景 | Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4). |
| 存放说明 | -20C |
| 计算分子量 | 200 |
参考图片Immunoprecipitation of HeLa cell lysates using Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423 (lane 1) and Rictor (D16H9) Rabbit mAb (Sepharose Bead Conjugate) (lane 2). The western blot was probed using Rictor (D61H9) Rabbit mAb #9476. 免疫沉淀分析HeLa 细胞提取物，所用抗体为Rabbit (DA1E) mAb IgG XP™ Isotype Control (Sepharose Bead Conjugate) #3423 (泳道 1)和Rictor (D16H9) Rabbit mAb (Sepharose Bead Conjugate)兔单抗 (泳道 2). Western blot采用Rictor (D61H9) Rabbit mAb 兔单抗#9476进行。 |
