| 货号 | 57706S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 目标/特异性 | Phospho-YAP (Ser127) (D9W2I) Rabbit mAb (Biotinylated) recognizes endogenous levels of YAP protein only when phosphorylated at Ser127. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5,6). In its capacity as a transcriptional co-activator, YAP is now widely recognized as a central mediator of the Hippo Pathway, which plays a fundamental and widely conserved role in regulating tissue growth and organ size. Upon phosphorylation at Ser127 by LATS1/2 kinases, YAP translocates to the cytoplasm, where it is sequestered through association with 14-3-3 proteins in an Akt-dependent manner (7-9). |
| 存放说明 | -20C |
| 计算分子量 | 65-75 |
| 参考文献 | 1 . Sudol, M. (1994) Oncogene 9, 2145-52. 2 . Mohler, P.J. et al. (1999) J Cell Biol 147, 879-90. 3 . Espanel, X. and Sudol, M. (2001) J Biol Chem 276, 14514-23. 4 . Sudol, M. et al. (1995) FEBS Lett 369, 67-71. 5 . Yagi, R. et al. (1999) EMBO J 18, 2551-62. 6 . Basu, S. et al. (2003) Mol Cell 11, 11-23. 7 . Zhao, B. et al. (2010) Genes Dev 24, 862-74. 8 . Zhao, B. et al. (2007) Genes Dev 21, 2747-61. 9 . Yu, F.X. et al. (2012) Cell 150, 780-91. 10 . Zhao, B. et al. (2010) Genes Dev 24, 72-85. |
Western blot analysis of extracts from PANC-1 cells, untreated (-) or λ-phosphatase-treated (+), using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb (Biotinylated). |
