| 货号 | 60388S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 目标/特异性 | HER2/ErbB2 (29D8) Rabbit mAb (HRP Conjugate) detects endogenous levels of total ErbB2 protein. This antibody does not cross-react with related kinases. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5). |
| 存放说明 | -20C |
| 计算分子量 | 185 |
| 参考文献 | 1 . Muthuswamy, S.K. et al. (1999) Mol Cell Biol 19, 6845-57. 2 . Qian, X. et al. (1994) Proc Natl Acad Sci USA 91, 1500-4. 3 . Dittadi, R. and Gion, M. (2000) J Natl Cancer Inst 92, 1443-4. 4 . Klapper, L.N. et al. (2000) Cancer Res 60, 3384-8. 5 . Kwon, Y.K. et al. (1997) J Neurosci 17, 8293-9. |
Western blot analysis of SK-BR-3 and MDA-MB-453 cell extracts using HER2/ErbB2 (29D8) Rabbit mAb (HRP Conjugate). |
