| 货号 | 66546S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 目标/特异性 | cGAS (D1D3G) Rabbit mAb (Biotinylated) recognizes endogenous levels of total cGAS protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Cyclic GMP-AMP synthase (cGAS, MB21D1) is an antiviral enzyme that produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (1,2). The cGAS protein acts as a cytosolic DNA sensor that binds DNA and produces the cGAMP second messenger from ATP and GTP (1,2). cGAMP binds to and activates STING, a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1-4). STING is ubiquitously expressed and found predominantly in the ER (3). Following activation, STING translocates with TBK1 to perinuclear endosomes (5). The TBK1 kinase phosphorylates and activates interferon regulatory factors (IRFs) and NF-κB, which leads to the induction of type I interferon and other immune response genes (3-5). |
| 存放说明 | -20C |
| 计算分子量 | 62 |
| 参考文献 | 1 . Sun, L. et al. (2013) Science 339, 786-91. 2 . Wu, J. et al. (2013) Science 339, 826-30. 3 . Ishikawa, H. and Barber, G.N. (2008) Nature 455, 674-8. 4 . Zhong, B. et al. (2008) Immunity 29, 538-50. 5 . Ishikawa, H. et al. (2009) Nature 461, 788-92. |
Western blot analysis of extracts from THP-1 and HeLa cell lines using cGAS (D1D3G) Rabbit mAb (Biotinylated). |
