| 货号 | 94994S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M R |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IF-IC F |
| 目标/特异性 | Phospho-Stat3 (Ser727) (D8C2Z) Rabbit mAb recognizes endogenous levels of Stat3 protein only when phosphorylated at Ser727. |
| 使用方法 | WB(1:1000) IP (1:200) F (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 86 |
| 参考文献 | 1 . Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120. 2 . Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4. 3 . Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15. 4 . Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85. 5 . Bromberg, J.F. et al. (1999) Cell 98, 295-303. 6 . Darnell, J.E. et al. (1994) Science 264, 1415-21. 7 . Ihle, J.N. (1995) Nature 377, 591-4. 8 . Wen, Z. et al. (1995) Cell 82, 241-50. 9 . Yokogami, K. et al. (2000) Curr Biol 10, 47-50. 10 . Biethahn, S. et al. (1999) Exp Hematol 27, 885-94. |
Confocal immunofluorescent analysis of A-431 (left, center; positive) and PC-3 (right; negative) cells, serum-starved and treated with Human Epidermal Growth Factor (hEGF) #8916 (100 ng/mL, 30 minutes; left, right) or Wortmannin #9915 (1 uM) and U0126 #9903 (10 uM, 1.5 hours; center) using Phospho-Stat3 (Ser727) (D8C2Z) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). | |
Western blot analysis of extracts from serum-starved A-431 cells, untreated (-) or treated with Human Epidermal Growth Factor #8916 (hEGF; 100ng/ml, 30 min; +) and λ-Phosphatase (λPPase; +) using Phospho-Stat3 (Ser727) (D8C2Z) XP® Rabbit mAb (upper) or total Stat3 (D3Z2G) Rabbit mAb #12640 (middle) or β-Actin (D6A8) Rabbit mAb #8457 (lower). PC-3 cells, which do not express Stat3, was used as a negative control. | |
Western blot analysis of extracts from serum-starved U266 cells, untreated (-) or treated with Human Interferon-α1 #8927 (hIFN-α1; 50ng/ml, 15 min; +), Hela cells, untreated (-) or treated with Human Tumor Necrosis Factor-α #8902 (hTNF-α; 20ng/ml, 30 min; +), HT-29 cells growing asynchronously (-) or arrested in mitosis by treatment with Thymidine (2mM, 17 hours) followed by Nocodazole #2190 (100ng/ml, 24 hrs; +), and NIH/3T3 cells, untreated (-) or treated with Human Platelet Derived Growth Factor-AA #8913 (hPDGF-AA; 50ng/ml, 30min; +) using Phospho-Stat3 (Ser727) (D8C2Z) Rabbit mAb (upper) or total Stat3 (124H6) Mouse mAb #9139 (lower). | |
Immunoprecipitation of Phospho-Stat3 (Ser727) from A-431 cells treated with Human Epidermal Growth Factor #8916 (100ng/ml 30min). Lane1 is 10% input, lane 2 is immunoprecipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-Stat3 (Ser727) (D8C2Z) XP® Rabbit mAb. Western blot was performed using Phospho-Stat3 (Ser727) (D8C2Z) XP® Rabbit mAb. | |
Flow cytometric analysis of human peripheral blood mononuclear cells, untreated (blue) or TPA-treated #4174 (40nM, 15 minutes; green), using Phospho-Stat3 (Ser727) (D8C2Z) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) was used as a secondary antibody. |
