| 货号 | 20194S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M R |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IF-F IF-IC |
| 目标/特异性 | Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) recognizes endogenous levels of tau protein when phosphorylated at Ser404. This antibody detects single phosphorylation at Ser404, dual phosphorylation at Ser400/Ser404 or Thr403/Ser404, and triple phosphorylation at Ser400/Thr403/Ser404. This antibody does not detect peptides with single phosphorylation at Ser400 or Thr403, and dual phosphorylation at Ser400/Thr403. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:200) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimers disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 50-80 |
| 参考文献 | 1 . Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9. 2 . Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76. 3 . Bramblett, G.T. et al. (1993) Neuron 10, 1089-99. 4 . Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76. 5 . Shiurba, R.A. et al. (1996) Brain Res 737, 119-32. 6 . Evans, D.B. et al. (2000) J Biol Chem 275, 24977-83. 7 . Bertrand, J. et al. (2010) Neuroscience 168, 323-34. |
Confocal immunofluorescent analysis of mouse primary neurons using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (green). Blue pseudocolor = Hoescht 33342 #4082 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of Tg2576 mouse brain, untreated (left) or Lambda Protein Phosphatase-treated (right), using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (red) and GFAP (GA5) Mouse mAb #3670 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from human cortex (lane 1), neonatal mouse brain, untreated (lane 2) or phosphatase-treated (lane 3), and fetal rat brain (lane 4), using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (upper), Tau (Tau46) Mouse mAb #4019 (middle) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from MEF cells (lane 1) and mouse brain (lane 2) using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (upper), Tau (Tau46) Mouse mAb #4019 (middle) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
